Elettra-Sincrotrone Trieste S.C.p.A. website uses session cookies which are required for users to navigate appropriately and safely. Session cookies created by the Elettra-Sincrotrone Trieste S.C.p.A. website navigation do not affect users' privacy during their browsing experience on our website, as they do not entail processing their personal identification data. Session cookies are not permanently stored and indeed are cancelled when the connection to the Elettra-Sincrotrone Trieste S.C.p.A. website is terminated.
More info
OK

Enzymes in dsDNA confined monolayers

We investigated the action of enzymes in crowded environment. Combining AFM and Molecular Dynamics simulations we were able to demonstrate that in DNA confined monolayers, restriction enzymes are inhibited or allowed to act depending on DNA sequence confromation and restriction site position along the chain.  C. Rotella et al., Nanoscale 9, 6399 (2017)      






 

Density-tunable nanografted monolayers (NAMs) of short oligonucleotide sequences on gold surfaces show novel properties that make them suitable for advanced biosensing applications, and in particular to study the effects of crowding and confinement on biomolecular interactions. Here, combining atomic force microscopy nanolithography, topography measurements and coarse-grained molecular dynamics simulations, we investigated restriction enzyme reaction mechanisms within confined DNA brushes highlighting the role played by the DNA sequence conformation and restriction site position along the chain, respectively, in determining the accessibility of the enzyme, and its consequent cleavage efficiency.

 

Retrieve article

Site accessibility tailors DNA cleavage by restriction enzymes in DNA confined monolayers

C. Rotella, G. Doni, A. Bosco, M. Castronovo, A. De Vita, L. Casalis, G.M. Pavan, P. Parisse
Nanoscale 9, 6399 (2017)
Ultima modifica il Lunedì, 12 Febbraio 2018 15:48