Life Sciences and cultural heritage Figure 1. (a) Representations of arbitrary orthogonal planes within the imaged OVECs supplemented tissue. Red arrows point out healthy follicles within a well-preserved tissue. (b) Zoomed image of the two follicles. (c) H&E slice of ovarian tissue grafted with OVECs-supplementation, showing OVECs forming perfused vessels, within the bovine scaffold matrix in the OVECs-supplemented tissue. (d) X-ray phase-contrast microCT virtual slice of ovarian tissue grafted without OVECs-supplementation. (e) H&E slice of ovarian tissue grafted without OVECs-supplementation. (f) X-ray phase-contrast microCT virtual slice of ovarian tissue grafted without OVECs-supplementation. (g) OVECs-supplemented iodine-stained tissue virtual section obtained by maximum intensity projection of selected x-ray phase-contrast microCT slices. M. Spazzapan1, S. Pegoraro2, R. Vuerich1,3, G. Zito2, A. Balduit2, E. Longo4 L. Pascolo2, M. Toffoli1, G. Meshini5, A. Mangogna2, G. Ros6, F. Buonomo2 F. Romano2, L. Lombardelli7, G. Papa5, M.P. Piccinni7, S. Zacchigna1,3 C. Agostinis2, R. Bulla1, G. Ricci2 1 Department of Life Sciences, University of Trieste, Trieste, Italy 2 Institute for Maternal and Child Health, IRCCS Burlo Garofolo, Trieste, Italy 3 Cardiovascular Biology Laboratory, International Centre for Genetic Engineering and Biotechnology (ICGEB), Trieste, Italy 4 Elettra-Sincrotrone Trieste SCpA, Basovizza, Trieste, Italy 5 Department of Medical, Surgical and Health Science, University of Trieste, Trieste, Italy 6 Central RNA Laboratory, Istituto Italiano di Tecnologia (IIT), Genova, Italy 7 Department of Experimental and Clinical Medicine, University of Florence, Florence, Italy e-mail: cagostinis@units.it Acknowledgments This work was supported by the Ministry of Health, Rome - Italy, in collaboration with the Institute for Maternal and Child Health IRCCS Burlo Garofolo, Trieste – Italy (RC23/18 and RC20/23 to G.R.), by the National Recovery and Resilience Plan (NRRP), funded by the European Union - Next Generation EU (CUP J53D23001020006 to R.B.), and by PON Research and Innovation Program (REACT EU 2014-2020) for the financial support provided to M.S.’s PhD. We acknowledge CERIC-ERIC (granted proposal 20237180) and EuroBioImaging (www.eurobioimaging.eu) for providing access to Elettra Sincrotrone Trieste. Original paper M. Spazzapan et al., Bioact. Mater., 50, 305 (2025), DOI: 10.1016/j.bioactmat.2025.03.021. Fig. 1 a c e g d f b Elettra Highlights 2024–2025 61
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