Life Sciences and cultural heritage Figure 1. (a) Scheme of deep penetration of tiny particles into our lungs and their accumulation on lung epithelial cells; (b) Novel multimodal correlative microscopy with the typical imaging ranges for each technique enables a comprehensive structural and functional investigation of the studied nanobio interface across a broad spatial scale, from tens of microns down to the nanometer level; (c) Correlated high-resolution CLSM, FLIM, SR µXRF and HIM providing an in-depth assessment of the nano-features and the related physicochemical effects on the TiO2-bio interface; (d) CLEM revealing the early formation of fibrin-like fibrous networks, a sign of acute cellular reaction to the nanomaterial clustered on the cell surface. Adaptation with permission, licensed under CC-BY 4.0 © 2025 The Authors. Published by American Chemical Society. Rok Podlipec1,2, Luka Pirker1,3, Ana Krišelj1, Gregor Hlawacek2 Alessandra Gianoncelli4, Primož Pelicon1 1 Jožef Stefan Institute, Ljubljana, Slovenia 2 Institute for Ion Beam Physics and Materials Research, Helmholtz-Zentrum Dresden-Rossendorf e.V., Dresden, Germany 3 Department of Electrochemical Materials, J. Heyrovský Institute of Physical Chemistry, v.v.i. Prague, Czech Republic 4 Elettra - Sincrotrone Trieste S.C.p.A., Trieste, Italy e-mail: rok.podlipec@ijs.si Acknowledgments The work received support from Helmholtz European Partnering project CROSSING (Grant No: PIE-0007), EU H2020 project no. 824096 “RADIATE”, the Slovenian research and innovation agency (ARIS) programs P1-0060 (Experimental biophysics of complex systems), P1-0112, I0-0005 and project N1-0090. We acknowledge Elettra Sincrotrone Trieste and in particular TwinMic beamline for providing access to its synchrotron radiation facilities. Original paper R. Podlipec, et al. ACS Nano 19, 18227 (2025); DOI: 10.1021/acsnano.4c17838. Fig. 1 Figure 1_Elettra Highlights-1.pdf 4250 x 2802 Elettra Highlights 2024–2025 67
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