XRD2 Publications

2021

  1. A Blueprint for High Affinity SARS-CoV-2 Mpro Inhibitors from Activity-Based Compound Library Screening Guided by Analysis of Protein Dynamics
    Gossen Jonas, Albani Simone, Hanke Anton, Joseph Benjamin P., Bergh Cathrine, Kuzikov Maria, Costanzi Elisa, Manelfi Candida, Storici P., Gribbon Philip, Beccari Andrea R., Talarico Carmine, Spyrakis Francesca, Lindahl Erik, Zaliani Andrea, Carloni Paolo, Wade Rebecca C., Musiani Francesco, Kokh Daria B., Rossetti Giulia
    ACS Pharmacology and Translational Science, Vol. 4 - 3, pp. 1079-1095 (2021)
    doi: 10.1021/acsptsci.0c00215 (Journal Article)
  2. An original 3D coordination polymer constructed from trinuclear nodes and tetracarboxylato spacers
    Dinca Alina S., Dogaru Andreea, Ion Adrian E., Nica Simona, Dumitrescu D., Shova Sergiu, Lloret Francesc, Julve Miguel, Andruh Marius
    CrystEngComm, Vol. 23 - 6, pp. 1332-1335 (2021)
    doi: 10.1039/D0CE01667E (Journal Article)
  3. Anti-trypanosomatidal drug discovery: a challenge for structural biology
    Battista T.
    (2021)
    (Thesis)
  4. Binding of dipeptidyl peptidase III to the oxidative stress cell sensor Kelch-like ECH-associated protein 1 is a two-step process
    Matić Sara, Kekez I., Tomin Marko, Bogár Ferenc, Šupljika Filip, Kazazić Saša, Hanić Maja, Jha Shalinee, Brkić Hrvoje, Bourgeois Benjamin, Madl Tobias, Gruber Karl, Macheroux Peter, Matković-Čalogović Dubravka, Matovina Mihaela, Tomić Sanja
    Journal of Biomolecular Structure and Dynamics, Vol. 39 - 18, pp. 6870-6881 (2021)
    doi: 10.1080/07391102.2020.1804455 (Journal Article)
  5. Chalcogenides-incorporating carbonic anhydrase inhibitors concomitantly reverted oxaliplatin-induced neuropathy and enhanced antiproliferative action
    Tanini Damiano, Carradori Simone, Capperucci Antonella, Lupori Lucrezia, Zara Susi, Ferraroni Marta, Ghelardini Carla, Mannelli LDC, Micheli Laura, Lucarini Elena, Carta Fabrizio, Angeli A., Supuran Claudiu T.
    European Journal of Medicinal Chemistry, Vol. 225, p. 113793 (2021)
    doi: 10.1016/j.ejmech.2021.113793 (Journal Article)
  6. Cold Crystallization of the Organic n-Type Small Molecule Semiconductor 2-Decyl-7-phenyl-[1]benzothieno[3,2-b][1]benzothiophene S,S,S′,S′-Tetraoxide
    Bodlos Wolfgang Rao, Mattiello Sara, Perinot Andrea, Gigli L., Demitri N., Beverina Luca, Caironi Mario, Resel Roland
    Crystal Growth and Design, Vol. 21 - 1, pp. 325-332 (2021)
    doi: 10.1021/acs.cgd.0c01157 (Journal Article)
  7. Colorimetric recognition of multiple first-row transition metals: a single water-soluble chemosensor in acidic and basic conditions
    Diana Rosita, Caruso Ugo, Di Costanzo L., Gentile Francesco Silvio, Panunzi Barbara
    Dyes and Pigments, Vol. 184, p. 108832 (2021)
    doi: 10.1016/j.dyepig.2020.108832 (Journal Article)
  8. Coordination versus hydrogen bonds in the structures of different tris(pyridin-2-yl)phosphoric triamide derivatives
    Sebghati Marjan, Tarahhomi Atekeh, Bozorgvar Marjan Sadat, Dumitrescu D.G., van der Lee Arie
    RSC Advances, Vol. 11 - 14, pp. 8178-8197 (2021)
    doi: 10.1039/D0RA10539B (Journal Article)
  9. Crystal structure and functional characterization of an oligosaccharide dehydrogenase from Pycnoporus cinnabarinus provides insights into fungal breakdown of lignocellulose
    Cerutti Gabriele, Gugole Elena, Montemiglio Linda Celeste, Turbé-Doan Annick, Chena Dehbia, Navarro David, Lomascolo Anne, Piumi François, Exertier Cécile, Freda Ida, Vallone B., Record Eric, Savino C., Sciara Giuliano
    Biotechnology for Biofuels, Vol. 14 - 1, p. 161 (2021)
    doi: 10.1186/s13068-021-02003-y (Journal Article)
  10. Development of potent dual PDK1/AurA kinase inhibitors for cancer therapy: Lead-optimization, structural insights, and ADME-Tox profile
    Sestito Simona, Bacci Andrea, Chiarugi Sara, Runfola Massimiliano, Gado Francesca, Margheritis Eleonora, Gul Sheraz, Riveiro Maria E., Vazquez Ramiro, Huguet Samuel, Manera Clementina, Rezai Keyvan, Garau G., Rapposelli Simona
    European Journal of Medicinal Chemistry, Vol. 226, p. 113895 (2021)
    doi: 10.1016/j.ejmech.2021.113895 (Journal Article)
  11. Exo-selective intermolecular Diels–Alder reaction by PyrI4 and AbnU on non-natural substrates
    Kashyap Rajnandani, Yerra Naga Veera, Oja Joachyutharayalu, Bala Sandeepchowdary, Potuganti Gal Reddy, Thota Jagadeshwar Reddy, Alla Manjula, Pal Debnath, Addlagatta A.
    Communications Chemistry, Vol. 4 - 1, p. 113 (2021)
    doi: 10.1038/s42004-021-00552-9 (Journal Article)
  12. H-Bonding and intramolecular catalysis of proton exchange affect the CEST properties of EuIII complexes with HP-DO3A-like ligands
    Baroni Simona, Carnovale Irene Maria, Carrera Carla, Boccalon Mariangela, Guidolin Nicol, Demitri Nicola, Lattuada Luciano, Tedoldi Fabio, Baranyai Zsolt, Aime Silvio
    Chemical Communications, Vol. 57 - 26, pp. 3287-3290 (2021)
    doi: 10.1039/D1CC00366F (Journal Article)
  13. How a Fragment Draws Attention to Selectivity Discriminating Features between the Related Proteases Trypsin and Thrombin
    Sandner Anna, Ngo Khang, Schiebel Johannes, Pizarroso Angela Ilse Marca, Schmidt Linda, Wenzel Benjamin, Steinmetzer Torsten, Ostermann Andreas, Heine Andreas, Klebe Gerhard
    Journal of Medicinal Chemistry, Vol. 64 - 3, pp. 1611-1625 (2021)
    doi: 10.1021/acs.jmedchem.0c01809 (Journal Article)
  14. Identification of Inhibitors of SARS-CoV-2 3CL-Pro Enzymatic Activity Using a Small Molecule in Vitro Repurposing Screen
    Kuzikov Maria, Costanzi Elisa, Reinshagen Jeanette, Esposito Francesca, Vangeel Laura, Wolf Markus, Ellinger Bernhard, Claussen Carsten, Geisslinger Gerd, Corona Angela, Iaconis Daniela, Talarico Carmine, Manelfi Candida, Cannalire Rolando, Rossetti Giulia, Gossen Jonas, Albani Simone, Musiani Francesco, Herzog Katja, Ye Yang, Giabbai Barbara, Demitri N., Jochmans Dirk, Jonghe Steven De, Rymenants Jasper, Summa Vincenzo, Tramontano Enzo, Beccari Andrea R., Leyssen Pieter, Storici P., Neyts Johan, Gribbon Philip, Zaliani Andrea
    ACS Pharmacology and Translational Science, Vol. 4 - 3, pp. 1096-1110 (2021)
    doi: 10.1021/acsptsci.0c00216 (Journal Article)
  15. Identification of a BAZ2A Bromodomain Hit Compound by Fragment Joining
    Dalle Vedove A., Cazzanelli Giulia, Corsi Jessica, Sedykh Maria, D'Agostino Vito Giuseppe, Caflisch Amedeo, Lolli G.
    ACS Bio and Med Chem Au, Vol. 1 - 1, pp. 5-10 (2021)
    doi: 10.1021/acsbiomedchemau.1c00016 (Journal Article)
  16. Imidazo[1,5-a]pyridine-3-ylidenes and dipyridoimidazolinylidenes as ancillary ligands in Palladium allyl complexes with potent in vitro anticancer activity
    Scattolin Thomas, Andreetta Giovanni, Mauceri Matteo, Rizzolio Flavio, Demitri N., Canzonieri Vincenzo, Visentin Fabiano
    Journal of Organometallic Chemistry, Vol. 952, p. 122014 (2021)
    doi: 10.1016/j.jorganchem.2021.122014 (Journal Article)
  17. Inhibition of urease, a Ni-enzyme: the reactivity of a key thiol with mono- and di-substituted catechols elucidated by kinetic, structural and theoretical studies
    Mazzei L., Contaldo Umberto, Musiani Francesco, Cianci M., Bagnolini Greta, Roberti Marinella, Ciurli S.
    Angewandte Chemie - International Edition, Vol. 60 - 11, pp. 6029-6035 (2021)
    doi: 10.1002/anie.202014706 (Journal Article)
  18. LrpCBA pilus proteins of gut-dwelling Ligilactobacillus ruminis: crystallization and X-ray diffraction analysis
    Prajapati Amar, Palva Airi, von Ossowski Ingemar, Krishnan Vengadesan
    Acta Crystallographica Section F: Structural Biology Communications, Vol. 77 - 8, pp. 238-245 (2021)
    doi: 10.1107/S2053230X21007263 (Journal Article)
  19. Mechanisms of the Antiproliferative and Antitumor Activity of Novel Telomerase–Carbonic Anhydrase Dual-Hybrid Inhibitors
    Plyasova Anna A., Berrino Emanuela, Khan Irina I., Veselovsky Alexander V., Pokrovsky Vadim S., Angeli A., Ferraroni Marta, Supuran Claudiu T., Pokrovskaya Marina V., Alexandrova Svetlana S., Gladilina Yulia A., Sokolov Nikolay N., Hilal Abdullah, Carta Fabrizio, Zhdanov Dmitry D.
    Journal of Medicinal Chemistry, Vol. 64 - 15, pp. 11432-11444 (2021)
    doi: 10.1021/acs.jmedchem.1c00756 (Journal Article)
  20. Mechanochemical Synthesis and Physicochemical Characterization of Previously Unreported Praziquantel Solvates with 2-Pyrrolidone and Acetic Acid
    Zanolla Debora, Gigli L., Hasa Dritan, Chierotti Michele R., Arhangelskis Mihails, Demitri Nicola, Jones William, Voinovich Dario, Perissutti B.
    Pharmaceutics, Vol. 13 - 10, pp. 1-20 (2021)
    doi: 10.3390/pharmaceutics13101606 (Journal Article)
  21. Methionine Aminopeptidases with Short Sequence Inserts within the Catalytic Domain are Differentially Inhibited: Structural and biochemical studies of three proteins from Vibrio spp.
    Pillalamarri Vijaykumar, Reddy Chilakala Gangi, Bala Sandeep Chowdary, Jangam Aruna, Kutty Vinny Vinod, Addlagatta A.
    European Journal of Medicinal Chemistry, Vol. 209, p. 112883 (2021)
    doi: 10.1016/j.ejmech.2020.112883 (Journal Article)
  22. Molecular insights into substrate recognition and catalysis by phthalate dioxygenase from Comamonas testosteroni
    Mahto Jai Krishna, Neetu Neetu, Waghmode Bhairavnath, Kuatsjah Eugene, Sharma Monica, Sircar Debabrata, Sharma Ashwani Kumar, Tomar Shailly, Eltis Lindsay D., Kumar Pravindra
    Journal of Biological Chemistry, Vol. 297 - 6, p. 101416 (2021)
    doi: 10.1016/j.jbc.2021.101416 (Journal Article)
  23. Multiple nanocages of a cyanophage small heat shock protein with icosahedral and octahedral symmetries
    Biswas Sreeparna, Garg Priyanka, Dutta Somnath, Suguna Kaza
    Scientific Reports, Vol. 11 - 1, p. 21023 (2021)
    doi: 10.1038/s41598-021-00172-2 (Journal Article)
  24. Potassium-induced partial inhibition of lactoperoxidase: structure of the complex of lactoperoxidase with potassium ion at 2.20 Å resolution
    Singh P.K., Pandey Sadanand, Rani Chitra, Ahmad Nayeem, Viswanathan V., Sharma Pradeep, Kaur Punit, Sharma Sujata, Singh T.P.
    Journal of Biological Inorganic Chemistry, Vol. 26 - 1, pp. 149-159 (2021)
    doi: 10.1007/s00775-020-01844-6 (Journal Article)
  25. Probing the Surface of a Parasite Drug Target Thioredoxin Glutathione Reductase Using Small Molecule Fragments
    Fata Francesca, Silvestri Ilaria, Ardini Matteo, Ippoliti Rodolfo, Di Leandro Luana, Demitri N., Polentarutti M., Di Matteo Adele, Lyu Haining, Thatcher Gregory R.J., Petukhov Pavel A., Williams David L., Angelucci F.
    ACS Infectious Diseases, Vol. 7 - 7, pp. 1932-1944 (2021)
    doi: 10.1021/acsinfecdis.0c00909 (Journal Article)
  26. Processive Enzymes Kept on a Leash: How Cellulase Activity in Multienzyme Complexes Directs Nanoscale Deconstruction of Cellulose
    Zajki-Zechmeister Krisztina, Kaira Gaurav Singh, Eibinger Manuel, Seelich Klara, Nidetzky Bernd
    ACS Catalysis, Vol. 11 - 21, pp. 13530-13542 (2021)
    doi: 10.1021/acscatal.1c03465 (Journal Article)
  27. Producing natural vanilla extract from green vanilla beans using a β-glucosidase from Alicyclobacillus acidiphilus
    Delgado Lidia, Heckmann Christian M., De Benedetti Stefano, Nardini Marco, Gourlay L.J., Paradisi Francesca
    Journal of Biotechnology, Vol. 329, pp. 21-28 (2021)
    doi: 10.1016/j.jbiotec.2021.01.017 (Journal Article)
  28. Reactions with proteins of three novel anticancer platinum(II) complexes bearing N-heterocyclic ligands
    Sacco F., Tarchi M., Ferraro G., Merlino A., Facchetti G., Rimoldi I., Messori L., Massai L.
    International Journal of Molecular Sciences, Vol. 22 - 19, p. 10551 (2021)
    doi: 10.3390/ijms221910551 (Journal Article)
  29. Salt bridges at the subdomain interfaces of the adenylation domain and active-site residues of Mycobacterium tuberculosis NAD+-dependent DNA ligase A (MtbLigA) are important for the initial steps of nick-sealing activity
    Afsar M., Shukla A., Kumar N., Ramachandran R.
    Acta Crystallographica Section D: Structural Biology, Vol. 77, pp. 776-789 (2021)
    doi: 10.1107/S2059798321003107 (Journal Article)
  30. Selective inhibition of Helicobacter pylori methionine aminopeptidase by azaindole hydroxamic acid derivatives: Design, synthesis, in vitro biochemical and structural studies
    Bala Sandeepchowdary, Yellamanda Kalisha vali, Kadari Anilkumar, Ravinuthala Venkata.S.U., Kattula Bhavita, Singh Om V., Gundla Rambabu, Addlagatta A.
    Bioorganic Chemistry, Vol. 115, p. 105185 (2021)
    doi: 10.1016/j.bioorg.2021.105185 (Journal Article)
  31. Spectroscopic/Computational Characterization and the X-ray Structure of the Adduct of the VIVO–Picolinato Complex with RNase A
    Ferraro Giarita, Demitri N., Vitale Luigi, Sciortino Giuseppe, Sanna Daniele, Ugone Valeria, Garribba Eugenio, Merlino A.
    Inorganic Chemistry, Vol. 60 - 24, pp. 19098-19109 (2021)
    doi: 10.1021/acs.inorgchem.1c02912 (Journal Article)
  32. Structural Insights into Schistosoma mansoni Carbonic Anhydrase (SmCA) Inhibition by Selenoureido-Substituted Benzenesulfonamides
    Angeli A., Ferraroni Marta, Da'dara Akram A., Selleri Silvia, Pinteala Mariana, Carta Fabrizio, Skelly Patrick J., Supuran Claudiu T.
    Journal of Medicinal Chemistry, Vol. 64 - 14, pp. 10418-10428 (2021)
    doi: 10.1021/acs.jmedchem.1c00840 (Journal Article)
  33. Structural and Biochemical Analysis of the Dual Inhibition of MG-132 against SARS-CoV-2 Main Protease (Mpro/3CLpro) and Human Cathepsin-L
    Costanzi Elisa, Kuzikov Maria, Esposito Francesca, Albani Simone, Demitri Nicola, Giabbai Barbara, Camasta Marianna, Tramontano Enzo, Rossetti Giulia, Zaliani Andrea, Storici P.
    International Journal of Molecular Sciences, Vol. 22 - 21, p. 11779 (2021)
    doi: 10.3390/ijms222111779 (Journal Article)
  34. Structural and related studies on Mevo lectin from Methanococcus voltae A3. The first thorough characterisation of an archeal lectin and its interactions.
    Sivaji Nukathoti, Suguna Kaza, Surolia Avadhesha, Vijayan Mamannamana
    Glycobiology, Vol. 31 - 3, pp. 315-328 (2021)
    doi: 10.1093/glycob/cwaa063 (Journal Article)
  35. Structural basis of inhibition of a transporter from Staphylococcus aureus, NorC, through a single-domain camelid antibody
    Kumar Sushant, Athreya A., Gulati Ashutosh, Nair Rahul Mony, Mahendran Ithayaraja, Ranjan Rakesh, Penmatsa A.
    Communications Biology, Vol. 4 - 1, p. 836 (2021)
    doi: 10.1038/s42003-021-02357-x (Journal Article)
  36. Structural basis of norepinephrine recognition and transport inhibition in neurotransmitter transporters
    Pidathala Shabareesh, Mallela Aditya Kumar, Joseph Deepthi, Penmatsa A.
    Nature Communications, Vol. 12 - 1, p. 2199 (2021)
    doi: 10.1038/s41467-021-22385-9 (Journal Article)
  37. Structural basis of ubiquitination mediated by protein splicing in early Eukarya
    Chiarini Valerio, Fiorillo A., Camerini Serena, Crescenzi Marco, Nakamura Shin, Battista T., Guidoni Leonardo, Colotti Gianni, Ilari Andrea
    Biochimica et Biophysica Acta - General Subjects, Vol. 1865 - 5, p. 129844 (2021)
    doi: 10.1016/j.bbagen.2021.129844 (Journal Article)
  38. Structural characterization of Myxococcus xanthus MglC, a component of the polarity control system, and its interactions with its paralog MglB
    Kapoor Srajan, Kodesia Akriti, Kalidas Nidhi, Ashish , Thakur Krishan Gopal
    Journal of Biological Chemistry, Vol. 296, p. 100308 (2021)
    doi: 10.1016/j.jbc.2021.100308 (Journal Article)
  39. Structural insights into kinetoplastid coronin oligomerization domain and F-actin interaction
    Parihar Pankaj Singh, Singh Aastha, Karade Sharanbasappa Shrimant, Sahasrabuddhe Amogh Anant, Pratap J. Venkatesh
    Current Research in Structural Biology, Vol. 3, pp. 268-276 (2021)
    doi: 10.1016/j.crstbi.2021.10.002 (Journal Article)
  40. Structure and metal-binding properties of PA4063, a novel player in periplasmic zinc trafficking by Pseudomonas aeruginosa
    Fiorillo A., Battistoni Andrea, Ammendola Serena, Secli Valerio, Rinaldo Serena, Cutruzzola Francesca, Demitri Nicola, Ilari Andrea
    Acta Crystallographica Section D: Structural Biology, Vol. 77 - 11, pp. 1401-1410 (2021)
    doi: 10.1107/S2059798321009608 (Journal Article)
  41. Surface layer proteins of Lactobacillus acidophilus - a story of SlpA and SlpX
    Sagmeister Theo, Eder Markus, Grininger Christoph, Buhlheller Christoph, Vejzovic Djenana, Dordic Andela, Damisch Elisabeth, Millan Claudia, Uson Isabel, Pavkov-Keller Tea
    XXV IUCr Congress, Vol. 77 - a2, p. C854 (2021)
    doi: 10.1107/S0108767321088449 (Conference Proceedings)
  42. Synthesis and anticancer activity of Pt(0)-olefin complexes bearing 1,3,5-triaza-7-phosphaadamantane and N-heterocyclic carbene ligands
    Scattolin Thomas, Valente Giorgia, Luzietti Lara, Piva Michele, Demitri N., Lampronti Ilaria, Gambari Roberto, Visentin F.
    Applied Organometallic Chemistry, Vol. 35 - 12, p. e6438 (2021)
    doi: 10.1002/aoc.6438 (Journal Article)
  43. Synthesis, characterization and anticancer activity of palladium allyl complexes bearing benzimidazole-based N-heterocyclic carbene (NHC) ligands
    Scattolin Thomas, Piccin Andrea, Mauceri Matteo, Rizzolio Flavio, Demitri N., Canzonieri Vincenzo, Visentin F.
    Polyhedron, Vol. 207, p. 115381 (2021)
    doi: 10.1016/j.poly.2021.115381 (Journal Article)
  44. The crystal structure of the domain-swapped dimer of onconase highlights some catalytic and antitumor activity features of the enzyme
    Gotte Giovanni, Campagnari Rachele, Loreto Domenico, Bettin Ilaria, Calzetti Federica, Menegazzi Marta, Merlino A.
    International Journal of Biological Macromolecules, Vol. 191, pp. 560-571 (2021)
    doi: 10.1016/j.ijbiomac.2021.09.095 (Journal Article)
  45. The structural and functional characterization of Malus domestica double bond reductase MdDBR provides insights towards the identification of its substrates
    Caliandro R., Polsinelli Ivan, Demitri Nicola, Musiani Francesco, Martens Stefan, Benini Stefano
    International Journal of Biological Macromolecules, Vol. 171, pp. 89-99 (2021)
    doi: 10.1016/j.ijbiomac.2020.12.190 (Journal Article)
  46. Triapine Analogues and Their Copper(II) Complexes: Synthesis, Characterization, Solution Speciation, Redox Activity, Cytotoxicity, and mR2 RNR Inhibition
    Besleaga Iuliana, Stepanenko Iryna, Petrasheuskaya Tatsiana V., Darvasiova Denisa, Breza Martin, Hammerstad Marta, Marć Małgorzata A., Prado-Roller Alexander, Spengler Gabriella, Popović-Bijelić Ana, Enyedy Eva A., Rapta Peter, Shutalev Anatoly D., Arion Vladimir B.
    Inorganic Chemistry, Vol. 60 - 15, pp. 11297-11319 (2021)
    doi: 10.1021/acs.inorgchem.1c01275 (Journal Article)
  47. Trop2 forms a stable dimer with significant structural differences within the membrane-distal region as compared to epcam
    Pavšič M.
    International Journal of Molecular Sciences, Vol. 22 - 19, p. 10640 (2021)
    doi: 10.3390/ijms221910640 (Journal Article)
  48. Unusual structural features in the adduct of dirhodium tetraacetate with lysozyme
    Loreto D., Ferraro G., Merlino A.
    International Journal of Molecular Sciences, Vol. 22 - 3, pp. 1-16 (2021)
    doi: 10.3390/ijms22031496 (Journal Article)
  49. Zinc<sup>2+</sup> ion inhibits SARS-CoV-2 main protease and viral replication <em>in vitro</em>
    Panchariya Love, Khan Wajahat Ali, Kuila Shobhan, Sonkar Kirtishila, Sahoo Sibasis, Ghoshal Archita, Kumar Ankit, Verma Dileep Kumar, Hasan Abdul, Das Shubhashis, Thakur Jitendra K, Halder Rajkumar, Sunil Sujatha, Arockiasamy Arulandu
    bioRxiv, p. 2021.06.15.448551 (2021)
    doi: 10.1101/2021.06.15.448551 (Journal Article)
  50. Zinc2+ion inhibits SARS-CoV-2 main protease and viral replicationin vitro
    Panchariya L., Khan W.A., Kuila S., Sonkar K., Sahoo S., Ghoshal A., Kumar A., Verma D.K., Hasan A., Khan M.A., Jain N., Mohapatra A.K., Das S., Thakur J.K., Maiti S., Nanda R.K., Halder R., Sunil S., Arockiasamy A.
    Chemical Communications, Vol. 57 - 78, pp. 10083-10086 (2021)
    doi: 10.1039/D1CC03563K (Journal Article)
Last Updated on Friday, 24 September 2021 08:45