NanoInnovationLAB Highlights


AFM-based Nanoimmunoassays

The immobilization of bio-molecules (e.g. DNA, proteins, enzymes) on surfaces with controlled orientations is important in the development of most powerful and sensitive sensors. To this end, we make the use of an emerging Atomic force microscopy (AFM) based nanofabrication tool known as nanografting, that allows for the fabrication of ordered and packed patches of biomolecular monolayer of variable density on ultra-flat gold surfaces. These surface-bound nanostructures appear to be a good candidate for advancing our understanding of protein-protein and protein-nucleic acids interactions. The effect of the reaction (for instance the reaction of DNA with a complementary strand) will persuade changes in the length of the DNA molecules involved that can be accurately measured by AFM height and/or friction measurements.

 

In particular the lab has developed the expertise in AFM-based protein immobilization at the nanoscale through bioaffinity immobilization (e.g. NTA - His-tag system), Dna Directed Immobilization, covalent immobilization of protein. The nanofabricated intarfaces are then exploited for the investigation of  biochemical activity of proteins, to evidence bio-recognition phenomena in a broad dynamical range, and the study of protein protein and protein ligand interaction expecially in the field of neurodegenerative diseases



Retrieve articles

Toward multiprotein nanoarrays using nanografting and DNA directed immobilization of proteins, Bano, F., Fruk, L., Sanavio, B., Glettenberg, M., Casalis, L., Niemeyer, C.M., Scoles, G. , Nanoletters 9, 2614 (2009)

Oriented Immobilization of Prion Protein Demonstrated via Precise Interfacial Nanostructure Measurements, Barbara Sanavio, Denis Scaini, Christian Grunwald, Giuseppe Legname, Giacinto Scoles, and Loredana Casalis, ACS Nano 4, 6607 (2010)



Last Updated on Monday, 20 July 2020 14:36