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Expression-Enhanced Fluorescent Proteins Based on Enhanced Green Fluorescent Protein for Super-resolution Microscopy


The first ever crystal structures of a negative reversibly switchable fluorescent protein derived from Aequorea victoria - called rsGreen0.7 -was determined in both the “on”- and “off”-conformation and provided further insights into the mechanisms underlying the photochromism. During photoswitching several structural rearrangements such as a cis-trans isomerization, backbone shifts and changes in hydrogen bonding occur.

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Expression-Enhanced Fluorescent Proteins Based on Enhanced Green Fluorescent Protein for Super-resolution Microscopy; Duwé S., De Zitter E., Gielen V., Moeyaert B., Vandenberg W., Grotjohann T., Clays K.,
Jakobs S., Van Meervelt L., and Dedecker P., ACS NANO, Article ASAP (2015) 10.1021/acsnano.5b04129



Ultima modifica il Mercoledì, 08 Settembre 2021 14:57