Pharma and Biotech

DNA microchip

microlaboratoriThe so-called DNA microchip, a particular type of diagnostic chip, is a microarray consisting of a grid of sensors capable of recognising particular sequences of genetic material. Chips of this kind are employed in genetics to provide early diagnosis of illnesses such as Alzheimer's disease, AIDS and certain types of cancer.

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Applications of virtual histology in biomedical research

istologiaHigh-resolution micro-computed tomography has been used for 3D virtual histology and volumetric quantification, but long acquisition times and additional staining procedures are required.

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Optimization of formulation and manufacturing conditions for the efficient development of coated tablets

compresse rivestiteCoating of solid pharmaceutical dosage forms is considered a key part in pharmaceutical production. The most important functions of tablet coatings are protection of tablet core, containing the active pharmaceutical ingredients, and control of drug dissolution properties (which may involve rapid, timed, or sustained release). Manufacturing conditions during the coating process can affect the performance of coatings by inducing alterations in their thickness, structural uniformity, and defects.

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Infrared (FTIRM) and low energy X-ray fluorescence (LEXRF) microscopies correlative study on rare diseases

malattie rareOchronotic pigment is a hallmark of Alkaptonuria (AKU), an ultra-rare disease responsible of tissue degeneration leading to a severe arthropathy in comorbidity with amyloidosis. Nevertheless, conventional bio-assays have not yet clarified composition and pathophysiological role of pigment and its structural relationship with amyloids.

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Polymorphism of human telomeric quadruplexes with anticancer drugs: a multi-technique biophysical study

quadruplexIdentification of small molecules able to stabilize telomeric G-quadruplexes is an attractive target for cancer therapy. Knowledge of quadruplex folding and structural polymorphism is required for structure-based rational drug design.

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FTIR spectroscopy for an improvement of cell selection in assisted reproduction technologies

FertilitĂ Success of assisted reproduction technologies are highly related to selection of good quality oocytes and spermatozoa. Analyses on oocyte macromolecular structure are necessary to detect and better evaluate alterations caused by aging. On the other hand, sperm selection is usually based only on morphology, motility and concentration characteristics. Considering the many possible alterations, there is a great need for analytical approaches allowing more effective sperm selections.

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FTIR studies on secondary structures of fibrous proteins: Amide III

CollageneFolding defects of fibrous proteins are involved in many genetic pathologies, such as fibrosis, Alzheimer's and Creutzfeldt-Jakob Disease. In particular, type I collagen is the most abundant fibrous-collagen protein in human, playing an important role in mammalian tissues. Its secondary structure needs to be extensively studied as anomalous organizations of collagen networks are detected to be involved in several pathologies.

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Structural insights into the interactions between an enzyme and a hit compound for the discovery of drugs

identificazione di nuovi farmaci antimicrobiciAn innovative approach to treat bacterial infections is based on the design of new drugs capable of enhancing antibiotic activity by reducing the therapeutic dose of conventional antibiotics and minimizing resistance. It is necessary to identify compounds able to selectively target a key enzyme, such as the enzyme involved in the microbial reductive sulfur assimilation pathway.

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Correlation between microstructure and bioequivalence in Anti-HIV Drug

bioequivalenza

Solid-state characteristics of drugs can impact their dissolution behaviour and, as a consequence, their bioavailability, affecting, thus, the bioequivalence of generic drugs. Bioequivalence of a poor-soluble drug, such as the anti-HIV Efavirenz, needs to be assessed by studying polymorphism particle size distributions and microstructure.
 
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Multi-technique analysis of extracellular vesicles

Vescicole

Therapeutic applications of extracellular vesicles as molecular carriers need a better understanding of the effects manufacturing processes could have on biological functions.
 
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Definition of the right excipients in a formulation for active pharmaceutical ingredients (APIs) stability

Formulazione

Physical and chemical instability of APIs is the reason of many market recalls of products, due to unacceptable levels of their decomposition, which alter content uniformity of the final formulation. Excipients’ properties, such as hygroscopicity and microenvironmental acidity, can have a significant impact on API stability in a drug product. Incompatibility or interaction between API and excipients, during manufacture or shelf storage, need detailed investigation.
 
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Last Updated on Wednesday, 01 June 2022 14:44