XRD1 Highlights
- XRD1 Highlights
- C1–C4 alcohol–cavitand complexes
- Cuprate Superconductor
- Jack Bean Urease
- XRD1 Sample Changer
- Carbonic Anhydrase
- PDI8CN2
- Human Legumain
- Structure of Human NAPE-PLD
- beta-Chitin in Squid Pen
- Enhanced Green Fluorescent Protein
- Multitarget drug design strategy
- Hydrogen-bonded Organic Pigments
- Selectivity of CNG channels
- Polycyclic Aromatic Hydrocarbons
- Cisplatin Encapsulation within the Ferritin Nanocage
- Crystal structure of the earthworm toxin
- Chemistry at the protein–mineral interface in L-ferritin
- Porous N-doped graphene
- Sliding of the human DNA clamp PCNA
- S1′ Pocket of Thermolysin
- microbial NLP cytolysins
- Human ubiquitin
- Photosynthesis
- Nanoparticles
- Anode Materials
- Stone Materials
- Sensor humidity
- Xe shell
- OSC
- Peptide nanotubes
- Amyloid aggregates
- Perovskites optimization
- Hydrocarbons
- CO2 separation
- Flexibility
- All Pages
Page 20 of 36
The mechanism of sliding of the human DNA clamp PCNA
X-ray crystallography: the 2.8 Å crystal structure of the human PCNA homotrimer bound to a 10 bp DNA duplex. A close-up shows the detailed interactions between the DNA phosphates (yellow spheres) and a set of positively charged residues within the PCNA central channel.
De March, M. et al., Nat.Commun. 8, 13935 (2017) |
DNA sliding clamps are ring-shaped proteins that encircle the DNA and harbour the polymerases, the enzymes that replicate DNA, allowing the duplication of the genetic material. How DNA clamps move onto the DNA double helix has remained elusive due to the peculiar nature of the interaction between the clamp and DNA. By a combination of x-ray crystallography, nuclear magnetic resonance (NMR), and molecular dynamics (MD) data, we have elucidated the molecular mechanism of sliding of the human clamp PCNA on DNA, a spiral motion that keeps the orientation of PCNA competent for binding to the replicative polymerase. |
Retrieve Article
A structural and computational analysis reveals the mechanism of sliding of the human DNA clamp PCNA, a central player in DNA replication;
De March M, Merino N, Barrera-Vilarmau S, Crehuet R, Onesti S, Blanco FJ, De Biasio A,
Nat.Commun. 8, 13935 (2017); doi: 10.1038/ncomms13935
|
Last Updated on Monday, 22 May 2023 15:31
